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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Clima Temperado; Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
29/03/2021 |
Data da última atualização: |
01/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MEDEIROS, M. B. de; VALLS, J. F. M.; ABREU, A. G. de; HEIDEN, G.; RIBEIRO-SILVA, S.; JOSE, S. C. B. R.; SANTOS, I. R. I.; PASSOS, A. M. A. dos; BURLE, M. L. |
Afiliação: |
MARCELO BRILHANTE DE MEDEIROS, Cenargen; JOSE FRANCISCO MONTENEGRO VALLS, Cenargen; ALUANA GONCALVES DE ABREU, CNPAF; GUSTAVO HEIDEN, CPACT; SUELMA RIBEIRO-SILVA, Instituto Chico Mendes de Conservação da Biodiversidade; SOLANGE CARVALHO B ROVERI JOSE, Cenargen; IZULME RITA IMACULADA SANTOS, Cenargen; ALEXANDRE MARTINS ABDAO DOS PASSOS, CNPMS; MARILIA LOBO BURLE, Cenargen. |
Título: |
Status of the ex situ and in situ conservation of brazilian crop wild relatives of rice, potato, sweet potato, and finger millet: filling the gaps of germplasm collections. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Agronomy, v. 11, article 638, 2021. |
ISSN: |
2073-4395 |
DOI: |
https://doi.org/10.3390/agronomy11040638 |
Idioma: |
Inglês |
Conteúdo: |
This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled "Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives" supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O?Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa's ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country. MenosThis study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled "Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives" supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O?Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the to... Mostrar Tudo |
Palavras-Chave: |
Banco de genes; Conservação ex situ; Conservação in situ; Crop wild relatives; Gap analysis. |
Thesagro: |
Arroz; Banco de Germoplasma; Batata; Batata Doce; Genética Vegetal; Germoplasma; Milheto; Oryza Sativa; Variedade. |
Thesaurus Nal: |
Ex situ conservation; Gene banks; Germplasm; In situ conservation; Potatoes; Rice; Sweet potatoes. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222370/1/agronomy-11-00638-v2.pdf
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Marc: |
LEADER 03647naa a2200493 a 4500 001 2130919 005 2021-04-01 008 2021 bl uuuu u00u1 u #d 022 $a2073-4395 024 7 $ahttps://doi.org/10.3390/agronomy11040638$2DOI 100 1 $aMEDEIROS, M. B. de 245 $aStatus of the ex situ and in situ conservation of brazilian crop wild relatives of rice, potato, sweet potato, and finger millet$bfilling the gaps of germplasm collections.$h[electronic resource] 260 $c2021 520 $aThis study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled "Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives" supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O?Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa's ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country. 650 $aEx situ conservation 650 $aGene banks 650 $aGermplasm 650 $aIn situ conservation 650 $aPotatoes 650 $aRice 650 $aSweet potatoes 650 $aArroz 650 $aBanco de Germoplasma 650 $aBatata 650 $aBatata Doce 650 $aGenética Vegetal 650 $aGermoplasma 650 $aMilheto 650 $aOryza Sativa 650 $aVariedade 653 $aBanco de genes 653 $aConservação ex situ 653 $aConservação in situ 653 $aCrop wild relatives 653 $aGap analysis 700 1 $aVALLS, J. F. M. 700 1 $aABREU, A. G. de 700 1 $aHEIDEN, G. 700 1 $aRIBEIRO-SILVA, S. 700 1 $aJOSE, S. C. B. R. 700 1 $aSANTOS, I. R. I. 700 1 $aPASSOS, A. M. A. dos 700 1 $aBURLE, M. L. 773 $tAgronomy$gv. 11, article 638, 2021.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Florestas. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
13/03/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. |
Afiliação: |
Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF. |
Título: |
Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. |
DOI: |
10.1007/s11676-018-0777-4 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. |
Palavras-Chave: |
Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética. |
Thesagro: |
Eucalipto; Eucalyptus Urophylla. |
Thesaurus NAL: |
Genetic transformation; In vitro regeneration; Somaclonal variation. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02181naa a2200313 a 4500 001 2114265 005 2020-03-13 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-018-0777-4$2DOI 100 1 $aBETTENCOURT, G. M. de F. 245 $aAgrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.$h[electronic resource] 260 $c2020 520 $aAbstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. 650 $aGenetic transformation 650 $aIn vitro regeneration 650 $aSomaclonal variation 650 $aEucalipto 650 $aEucalyptus Urophylla 653 $aPlant growth regulators 653 $aRegeneração in vitro 653 $aReguladores de crescimento de plantas 653 $aTransformação genética 700 1 $aSOCCOL, C. R. 700 1 $aGIOVANELLA, T. S. 700 1 $aFRANCISCON, L. 700 1 $aKESTRING, D. R. 700 1 $aGERHARDT, I. R. 700 1 $aDEGENHARDT-GOLDBACH, J. 773 $tJournal of Forestry Research$gv. 31, n. 2, p. 507-519, Apr. 2020.
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